Velasco R, Salamini F, Bartels D
Max-Planck-Institut für Züchtungsforschung, Köln, Germany.
Plant Mol Biol. 1994 Oct;26(1):541-6. doi: 10.1007/BF00039567.
We report the identification of a full-length cDNA clone encoding cytosolic glyceraldehyde-3-phosphate dehydrogenase (GAPDH, EC 1.2.1.12) in the desiccation-tolerant plant Craterostigma plantagineum. The DNA sequence of the cDNA clone is homologous to cytosolic GAPDH cDNAs from other higher plants. The GAPDH transcript increases rapidly in abundance during dehydration or abscisic acid (ABA) treatment. The increase in mRNA levels is directly correlated with higher protein and enzyme levels. These results imply that enhanced rates of glycolysis are one of the immediate cellular responses to water deficit. This may be a mechanism by which the plant cell prepares for a demand of ATP and NADH2 during recovery.
我们报道了在耐旱植物海角苣苔中鉴定出一个编码胞质甘油醛-3-磷酸脱氢酶（GAPDH，EC 1.2.1.12）的全长cDNA克隆。该cDNA克隆的DNA序列与其他高等植物的胞质GAPDH cDNA具有同源性。在脱水或脱落酸（ABA）处理过程中，GAPDH转录本的丰度迅速增加。mRNA水平的增加与更高的蛋白质和酶水平直接相关。这些结果表明，糖酵解速率的提高是细胞对水分亏缺的即时反应之一。这可能是植物细胞在恢复过程中为满足ATP和NADH2需求而准备的一种机制。
