Mitra A, Higgins D W
Department of Plant Pathology, University of Nebraska, Lincoln 68583-0722.
Plant Mol Biol. 1994 Oct;26(1):85-93. doi: 10.1007/BF00039522.
An upstream region isolated from a eukaryotic algal virus adenine methyltransferase gene was tested for promoter function in plants. Fusion of this region to the chloramphenicol acetyltransferase reporter gene resulted in significantly higher expression than fusion with the cauliflower mosaic virus 35S promoter. Strong levels of expression were also found in electroporated monocot plant cells. The promoter activity in transgenic tobacco plants showed tissue-specific expression. Leaves had the highest expression followed by stems and flowers. The promoter activity was not detected in root tissue. Environmental cues, such as light, and the phytohormones auxin and cytokinines had no effect on the promoter's expression. This promoter might be utilized to achieve high levels of expression of introduced genes in higher plants.
对从真核藻类病毒腺嘌呤甲基转移酶基因分离出的上游区域进行了植物启动子功能测试。该区域与氯霉素乙酰转移酶报告基因融合后，表达水平显著高于与花椰菜花叶病毒35S启动子融合的情况。在电穿孔的单子叶植物细胞中也发现了高水平的表达。转基因烟草植株中的启动子活性表现出组织特异性表达。叶片中的表达最高，其次是茎和花。在根组织中未检测到启动子活性。光照等环境信号以及植物激素生长素和细胞分裂素对启动子的表达没有影响。该启动子可用于在高等植物中实现导入基因的高水平表达。
