Lapolla A, Fedele D, Seraglia R, Catinella S, Traldi P
Department of Internal Medicine, University of Padua, Italy.
Rapid Commun Mass Spectrom. 1994 Aug;8(8):645-52. doi: 10.1002/rcm.1290080815.
Matrix-assisted laser desorption/ionization mass spectrometry has been successfully applied in the study of non-enzymatic glycation of different proteins. In the case of bovine serum albumin, glycated by in vitro experiments performed under pseudophysiological conditions, a clear increase in molecular weight is observed with respect to both glucose concentrations and incubation time. The in vitro glycation of ribonuclease with glucose and fructose shows some peculiar differences either in terms of the number of condensed sugar molecules or in terms of the reaction kinetics. The same approach, applied to plasma proteins of healthy and diabetic subjects, provides evidence for the occurrence of glycation of human serum albumin for the latter subjects.
基质辅助激光解吸/电离质谱已成功应用于不同蛋白质非酶糖基化的研究。以牛血清白蛋白为例，在伪生理条件下进行体外实验使其糖基化，相对于葡萄糖浓度和孵育时间，观察到分子量明显增加。核糖核酸酶与葡萄糖和果糖的体外糖基化在缩合糖分子数量或反应动力学方面均表现出一些独特差异。将同样的方法应用于健康受试者和糖尿病患者的血浆蛋白，为后者体内人血清白蛋白糖基化的发生提供了证据。
