Huang X, Xia T, Ren P
Xijing Hospital, Fourth Military Medical University, Xi'an.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 1994 May;14(5):288-91, 261-2.
Influence of Salvia miltiorrhiza (SM) and/or Ligusticum wallichii (LW) on pharmacokinetics of tetramethylpyrazine (TMP) was observed in rats. The content of TMP in LW and LW-SM decoctions were 375.8 and 236.2 micrograms/ml respectively. The rat serum components after oral administration of LW were analyzed by modified HPLC. One of 3 compounds was detected, which was identified as TMP by the comparison of the parameters of 4 spectrums (UV, IR, MS and NMR) with those of TMP in literature. Pharmacokinetics of TMP in rats after the oral administration of LW and LW-SM decoction respectively showed that: (1) both of the data fitted adequately to two-compartment open model; (2) Ka, AUC and serum concentration were higher (P < 0.05-0.01) for LW decoction than that for LW-SM decoction, indicating a higher bioavailability. It demonstrated that the absorption of TMP of LW-SM decoction was slower and the bioavailability of TMP of LW-SM decoction reduced. Contents of TMP in LW and LW-SM decoction and serum concentration of TMP were determined by HPLC method.
观察丹参（SM）和/或川芎（LW）对大鼠体内川芎嗪（TMP）药代动力学的影响。LW汤和LW - SM汤中TMP的含量分别为375.8和236.2微克/毫升。采用改良的高效液相色谱法分析大鼠口服LW后的血清成分。检测到3种化合物中的1种，通过将其4种光谱（紫外、红外、质谱和核磁共振）参数与文献中TMP的参数进行比较，鉴定为TMP。大鼠分别口服LW汤和LW - SM汤后TMP的药代动力学研究表明：（1）两者数据均充分拟合二室开放模型；（2）LW汤的Ka、AUC和血清浓度高于LW - SM汤（P < 0.05 - 0.01），表明生物利用度更高。结果表明，LW - SM汤中TMP的吸收较慢，LW - SM汤中TMP的生物利用度降低。采用高效液相色谱法测定LW和LW - SM汤中TMP的含量以及TMP的血清浓度。
