Yoshihara K, Mochidome N, Hara T, Osada S, Takayama A, Nagata M
Department of Public Health, Tokyo College of Pharmacy, Japan.
Biol Pharm Bull. 1994 Jun;17(6):836-9. doi: 10.1248/bpb.17.836.
The urinary excretion levels of hydroxylysine glycosides [Hyl-Gs; O-beta-D-Galactopyranosylhydroxylysine (GH) and 2-O-alpha-D-glucopyranosyl-O-beta-D-galactopyranosylhydroxylysine (GGH)], provide a new index of collagen metabolism. The determination of urinary hydroxylysine glycoside excretion levels was applied to 20 osteoporotic patients and 208 healthy control subjects (69 females and 139 males) in order to evaluate the conditions of bone collagen metabolism. Urinary Hyl-Gs were analyzed by the method of pre-column fluorescent derivatized HPLC. Compared to the age and sex-adjusted control, the urinary excretion levels of hydroxylysine glycosides, especially GH, in osteoporotic patients were significantly higher than in the age and sex-adjusted control, and the urinary GGH/GH ratio was lower. Sixteen of the 20 patients whose Hyl-Gs levels we followed exhibited significantly lower values after 200 d compared to the initial levels. These results suggest that clinical therapeutics affected the bone resorption and, therefore, demonstrated the usefulness of determining urinary excretion levels of Hyl-Gs for evaluating the conditions of bone metabolism.
羟赖氨酸糖苷[Hyl-Gs；O-β-D-吡喃半乳糖基羟赖氨酸（GH）和2-O-α-D-吡喃葡萄糖基-O-β-D-吡喃半乳糖基羟赖氨酸（GGH）]的尿排泄水平提供了一种新的胶原代谢指标。为了评估骨胶原代谢状况，对20例骨质疏松症患者和208名健康对照者（69名女性和139名男性）进行了尿羟赖氨酸糖苷排泄水平的测定。采用柱前荧光衍生化高效液相色谱法分析尿中的Hyl-Gs。与年龄和性别校正后的对照组相比，骨质疏松症患者尿中羟赖氨酸糖苷，尤其是GH的排泄水平显著高于年龄和性别校正后的对照组，且尿中GGH/GH比值较低。在我们跟踪其Hyl-Gs水平的20例患者中，有16例在200天后的数值与初始水平相比显著降低。这些结果表明临床治疗影响了骨吸收，因此，证明了测定尿中Hyl-Gs排泄水平对评估骨代谢状况的有用性。
