Díaz-Flores M, Duran-Reyes G, Hicks J J
Unidad de Investigación Médica en Bioquímica, Instituto Mexicano del Seguro Social México City.
Int J Fertil Menopausal Stud. 1994 Jul-Aug;39(4):234-8.
The purpose of this study was to investigate the activity of gamma-glutamyl transpeptidase (gamma-GTP, E.C. 2.3.2.2) in rat endometrium (day 5 of pregnancy). Since gamma-GTP is an enzyme involved in the translocation of amino acids from fluids toward tissues, these substrates are necessary for anabolic processes.
The presence of statistically higher activity of gamma-GTP in rat (Sprague-Dawley) implantation sites (1.06 nmol/mg protein/min) than in nondecidualized (0.87 nmol/mg protein/min) tissues was demonstrated. The intrauterine administration of L-serine-borate complex (5 mM) during day 5 of pregnancy arrested 91.6% of rat embryonic development (day 18). This inhibitory effect was not present when borate or L-serine was administered separately. The L-serine-borate complex also inhibited (by 88%) the gamma-GTP in vitro.
The inhibition of gamma-GTP by L-serine-borate complex might be considered as a new approach to the arrest of biological processes in differentiation or development.
本研究旨在调查大鼠子宫内膜（妊娠第5天）中γ-谷氨酰转肽酶（γ-GTP，E.C. 2.3.2.2）的活性。由于γ-GTP是一种参与氨基酸从液体向组织转运的酶，这些底物对于合成代谢过程是必需的。
结果表明，大鼠（斯普拉格-道利）着床部位的γ-GTP活性（1.06纳摩尔/毫克蛋白质/分钟）在统计学上高于未蜕膜化组织（0.87纳摩尔/毫克蛋白质/分钟）。在妊娠第5天经子宫内给予L-丝氨酸-硼酸盐复合物（5毫摩尔）可使91.6%的大鼠胚胎发育停滞（第18天）。单独给予硼酸盐或L-丝氨酸时不存在这种抑制作用。L-丝氨酸-硼酸盐复合物在体外也抑制（88%）γ-GTP。
L-丝氨酸-硼酸盐复合物对γ-GTP的抑制作用可被视为阻止分化或发育中的生物过程的一种新方法。
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