Ishimatsu M, Nakamura Y, Kato H
Department of Obstetrics and Gynecology, Yamaguchi University School of Medicine, Japan.
Endocr J. 1993 Dec;40(6):665-71. doi: 10.1507/endocrj.40.665.
Superovulation with exogenous gonadotropins has been used widely to study the reproductive events in animals and in vitro fertilization and embryo transfer in human beings. However, details of the mechanism of superovulation are not yet clearly understood. The present study was conducted to study the mechanism of superovulation with purified human FSH and LH preparations. Cyclic hamsters received continuous infusion of purified FSH and LH for 4 days, from estrus to proestrus. The number of ova shed was significantly increased by 5 iu FSH/minipump infusion, and further increased by 5 iu FSH/minipump plus 5 iu LH/minipump group. On the other hand, the number of atretic follicles was decreased significantly (P < 0.01) by the combined infusion of FSH and LH, but not by FSH alone. Among these experimental groups, there was no significant difference in embryonic development which was assessed by the counting of the number of 4-cells and more developed embryos on day 3 of pregnancy. These data indicated the different roles of FSH and LH in the superovulation process, because FSH stimulated some small follicles to grow and LH, in the presence of FSH, rescued the follicles to undergo atresia.
使用外源性促性腺激素进行超排卵已被广泛应用于研究动物的生殖过程以及人类的体外受精和胚胎移植。然而，超排卵的机制细节尚未完全明确。本研究旨在探讨使用纯化的人促卵泡激素（FSH）和促黄体生成素（LH）制剂进行超排卵的机制。处于发情周期的仓鼠从发情期到发情前期连续4天接受纯化的FSH和LH输注。通过微量泵以每分钟5国际单位的FSH输注可使排出的卵子数量显著增加，而在每分钟5国际单位FSH加每分钟5国际单位LH的微量泵输注组中卵子数量进一步增加。另一方面，FSH和LH联合输注可使闭锁卵泡数量显著减少（P < 0.01），而单独使用FSH则无此效果。在这些实验组中，通过计算妊娠第3天4细胞及更发育胚胎的数量来评估胚胎发育情况，各实验组之间无显著差异。这些数据表明FSH和LH在超排卵过程中发挥着不同作用，因为FSH刺激一些小卵泡生长，而LH在FSH存在的情况下挽救卵泡使其免于闭锁。
