使用聚乙二醇、胃蛋白酶和吐温80纯化乙型肝炎表面抗原。
Purification of hepatitis B surface antigen using polyethylene gylcol, pepsin and Tween 80.
作者信息
Nath N, Mazzur S, Ledman R, Fang C T
出版信息
Vox Sang. 1976;31(1 SUPPL):84-95. doi: 10.1111/j.1423-0410.1976.tb02226.x.
A simple, inexpensive procedure for the isolation and purification of HB8Ag from plasma is described. The technique included precipitation of HBsAg with PEG and elimination of normal plasma proteins by digestion with pepsin. Tween 80 was used to remove contaminating lipoprotein(s). This technique resulted in about a 200-fold gain in the specific activity of HBsAg and yielded about 20-40% recovery. Rabbits immunized with the purified antigen produced type-specific antibodies to HBsAg without detectable reactivity to normal human plasma antigens.
本文描述了一种从血浆中分离和纯化HB8Ag的简单、廉价的方法。该技术包括用聚乙二醇沉淀乙肝表面抗原（HBsAg），并用胃蛋白酶消化去除正常血浆蛋白。使用吐温80去除污染的脂蛋白。该技术使HBsAg的比活性提高了约200倍，回收率约为20%-40%。用纯化抗原免疫的兔子产生了针对HBsAg的型特异性抗体，对正常人血浆抗原无明显反应。
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