Nakajima T, Kagawa K, Ueda K, Ohkawara T, Kimura H, Kakusui M, Deguchi T, Okanoue T, Kashima K, Ashihara T
Third Department of Internal Medicine, Kyoto Prefectural University of Medicine, Japan.
J Gastroenterol. 1994 Aug;29(4):450-4. doi: 10.1007/BF02361242.
The proliferative activity of chronic liver diseases and hepatocellular carcinomas (HCCs) was studied by PCNA immunohistochemistry. Human liver tissues were obtained by surgical operation or needle biopsy, and PCNA was detected by immunohistochemistry. PCNA-labelling indices (PCNA-LIs) of methanol-fixed tissues corresponded with the incidence of S-phase cells previously reported, whereas paraformaldehyde-fixed tissues showed extremely high PCNA-LIs in all specimens. Therefore, methanol-fixed tissues were used for evaluation. The PCNA-LIs of the methanol-fixed tissues were: normal liver 0.78 +/- 0.38%, chronic persistent hepatitis 1.06 +/- 0.86%, chronic aggressive hepatitis 2A 1.01 +/- 0.50%, chronic aggressive hepatitis 2B 4.20 +/- 1.79%, inactive cirrhosis 0.81 +/- 0.49%, active cirrhosis 1.96 +/- 0.93%, HCC of Edmondson's type I 4.83 +/- 1.98%, type II 6.65 +/- 1.69%, and type III 38.7 +/- 30.6%. PCNA-positive cells showed little specific distribution; in periportal areas in chronic hepatitis, at the margins of pseudolobules in cirrhosis, and throughout the tumor in HCC. These findings indicated that proliferative activity increased during the progression of chronic hepatitis, but that it decreased at the stage of cirrhosis. In chronic liver diseases, the PCNA-LIs reflected hepatitis activity. HCC showed higher proliferative activity than liver cirrhosis, and the histological grade was correlated with the PCNA-LI.
通过增殖细胞核抗原（PCNA）免疫组织化学方法研究了慢性肝病和肝细胞癌（HCC）的增殖活性。通过手术操作或针吸活检获取人肝组织，并采用免疫组织化学方法检测PCNA。甲醇固定组织的PCNA标记指数（PCNA-LIs）与先前报道的S期细胞发生率相符，而多聚甲醛固定组织在所有标本中均显示出极高的PCNA-LIs。因此，采用甲醇固定组织进行评估。甲醇固定组织的PCNA-LIs分别为：正常肝脏0.78±0.38%，慢性持续性肝炎1.06±0.86%，慢性活动性肝炎2A 1.01±0.50%，慢性活动性肝炎2B 4.20±1.79%，静止性肝硬化0.81±0.49%，活动性肝硬化1.96±0.93%，Edmondson I型HCC 4.83±1.98%，II型6.65±1.69%，III型38.7±30.6%。PCNA阳性细胞未见特异性分布；在慢性肝炎的汇管区周围、肝硬化假小叶边缘以及HCC的整个肿瘤组织中均有分布。这些结果表明，增殖活性在慢性肝炎进展过程中增加，但在肝硬化阶段降低。在慢性肝病中，PCNA-LIs反映肝炎活动度。HCC显示出比肝硬化更高的增殖活性，且组织学分级与PCNA-LI相关。
