Shen Li-Juan, Zhang Hua-Xian, Zhang Zong-Ji, Li Jin-Yun, Chen Ming-Qin, Yang Wei-Bo, Huang Run
Department of Pathology, Kunming Medical College, Kunmming 650031, Yunnan Province, China.
World J Gastroenterol. 2003 Mar;9(3):459-62. doi: 10.3748/wjg.v9.i3.459.
To investigate the change of HBV DNA, PCNA and GST-pi in chronic liver disease and hepatocellular carcinoma (HCC).
Hepatitis B surface antigen (HBsAg), proliferating cell nuclear antigen (PCNA) and glutathione S-transferases (GST-pi) were detected by immunohistochemical staining and HBV DNA was detected by in situ hybridization (ISH) in formalin-fixed and paraffin-embedded sections with a total of 111 specimens of chronic hepatitis, liver cirrhosis, paratumorous tissue, HCC and normal liver tissue.
The positive rates of HBsAg and HBVDNA were 62.5 %(15/24) and 75.0 %(12/16) in chronic hepatitis, 64.0 %(16/25) and 83.3 %(15/18) in liver cirrhosis, 72.7 %(16/22) and 85.7 %(12/14) in the paratumorous tissu and 45.0 %(14/31) and 64.3 %(9/14) in HCC. The positive HBVDNA granules in chronic hepatitis, liver cirrhosis and the paratumorous tissue were more intense than that in HCC. The positive rates of PCNA and GST-pi were 34.8 %(8/23) and 25.0 %(4/16) in chronic hepatitis, 73.7 %(14/19) and 17.6 %(3/17) in liver cirrhosis, 86.7 %(13/15) and 53.3 %(8/15) in the paratumorous tissue, 100 %(15/15) and 60.0 %(9/15) in HCC, respectively, and the positive rate of GST-pi in the paratumorous tissue was significantly higher than that in the liver cirrhosis without tumor (P<0.05), but same as that in HCC (P>0.05).
The HBV infection may increase expression of PCNA and GST-pi. The paratumor cirrhosis may be a sequential lesion of precancerous cirrhosis around HCC.
研究慢性肝病及肝细胞癌（HCC）中乙肝病毒脱氧核糖核酸（HBV DNA）、增殖细胞核抗原（PCNA）和谷胱甘肽S转移酶π（GST-pi）的变化。
采用免疫组织化学染色法检测111例慢性肝炎、肝硬化、癌旁组织、HCC及正常肝组织标本中乙肝表面抗原（HBsAg）、增殖细胞核抗原（PCNA）和谷胱甘肽S转移酶（GST-pi），采用原位杂交（ISH）法检测HBV DNA，标本均为甲醛固定石蜡包埋切片。
慢性肝炎中HBsAg和HBV DNA阳性率分别为62.5%（15/24）和75.0%（12/16），肝硬化中分别为64.0%（16/25）和83.3%（15/18），癌旁组织中分别为72.7%（16/22）和85.7%（12/14），HCC中分别为45.0%（14/31）和64.3%（9/14）。慢性肝炎、肝硬化及癌旁组织中HBV DNA阳性颗粒较HCC更为明显。慢性肝炎中PCNA和GST-pi阳性率分别为34.8%（8/23）和25.0%（4/16），肝硬化中分别为73.7%（14/19）和17.6%（3/17），癌旁组织中分别为86.7%（13/15）和53.3%（8/15），HCC中分别为100%（15/15）和60.0%（9/15），癌旁组织中GST-pi阳性率显著高于无肿瘤的肝硬化组织（P<0.05），与HCC相同（P>0.05）。
HBV感染可能增加PCNA和GST-pi的表达。癌旁肝硬化可能是HCC周围癌前肝硬化的连续病变。
