Shiosaki A, Goto M, Hirose T
Department of Applied Chemistry, Kumamoto University, Japan.
J Chromatogr A. 1994 Sep 9;679(1):1-9. doi: 10.1016/0021-9673(94)80306-4.
The adsorption behaviour of two kinds of proteins, myoglobin and ovalbumin, with a membrane adsorber, DEAE MemSep 1000 (Millipore), was studied in comparison with a bead-based packed-bed adsorber, DEAE Sephacel (Pharmacia-LKB), by means of frontal analysis. Adsorption isotherms were obtained by integrating the breakthrough curves for various feed concentrations. Adsorption isotherms were expressed by the Langmuir equation and the adsorption capacity for the membrane adsorber was smaller than that for the packed-bed adsorber. The breakthrough curves of myoglobin for the membrane adsorber were independent of the flow-rate, but those of ovalbumin were affected by the flow-rate. Abnormal behaviour was observed for the adsorption of ovalbumin on a membrane adsorber. With the packed-bed adsorber, the breakthrough curves for both proteins were significantly affected by the flow-rate. A mathematical model for the membrane adsorber involving axial dispersion and adsorption kinetics was derived. The model simulated the breakthrough curves for myoglobin well. Axial dispersion was dominant for the membrane adsorber whereas intraparticle diffusion was dominant for the packed-bed adsorber.
通过前沿分析法，研究了两种蛋白质——肌红蛋白和卵清蛋白——在膜吸附剂DEAE MemSep 1000（密理博公司）上的吸附行为，并与基于珠子的填充床吸附剂DEAE Sephacel（法玛西亚-莱克勃公司）进行了比较。通过对不同进料浓度的穿透曲线进行积分，得到了吸附等温线。吸附等温线用朗缪尔方程表示，膜吸附剂的吸附容量小于填充床吸附剂的吸附容量。膜吸附剂上肌红蛋白的穿透曲线与流速无关，但卵清蛋白的穿透曲线受流速影响。观察到卵清蛋白在膜吸附剂上的吸附存在异常行为。对于填充床吸附剂，两种蛋白质的穿透曲线均受流速显著影响。推导了一个涉及轴向扩散和吸附动力学的膜吸附剂数学模型。该模型能很好地模拟肌红蛋白的穿透曲线。轴向扩散在膜吸附剂中占主导地位，而颗粒内扩散在填充床吸附剂中占主导地位。
