通过毛细管等速电泳法测定植酸酶对植酸（肌醇六磷酸）进行酶促水解过程中肌醇磷酸形成的时间进程。

Time course of formation of inositol phosphates during enzymatic hydrolysis of phytic acid (myo-inositol hexaphosphoric acid) by phytase determined by capillary isotachophoresis.

作者信息

Blatný P, Kvasnicka F, Kenndler E

机构信息

Institute of Analytical Chemistry, University of Vienna, Austria.

出版信息

J Chromatogr A. 1994 Sep 23;679(2):345-8. doi: 10.1016/0021-9673(94)80577-6.

DOI:10.1016/0021-9673(94)80577-6

PMID:7951996

Abstract

Capillary isotachophoresis with conductivity detection was applied to the investigation of the hydrolytic decomposition of phytic acid (myo-inositol hexaphosphoric acid) by phytase, and for the formation of the reaction products as a function of time. The quantitation of all analytes (besides phytic acid the mono- to penta-phosphorylated inositols and orthophosphate) can be carried out using two different buffer systems.

摘要

采用带电导检测的毛细管等速电泳法研究植酸酶对植酸（肌醇六磷酸）的水解分解作用以及反应产物随时间的形成情况。所有分析物（除植酸外，还有单磷酸至五磷酸化的肌醇和正磷酸盐）的定量分析可使用两种不同的缓冲体系进行。