毛细管区带电泳法测定中枢神经系统疾病患者脑脊液中的有机酸
Capillary zone electrophoretic determination of organic acids in cerebrospinal fluid from patients with central nervous system diseases.
作者信息
Hiraoka A, Akai J, Tominaga I, Hattori M, Sasaki H, Arato T
机构信息
Department of Mental Health, Kyorin University School of Health Sciences, Tokyo, Japan.
出版信息
J Chromatogr A. 1994 Sep 30;680(1):243-6. doi: 10.1016/0021-9673(94)80073-1.
Organic acids in cerebrospinal fluid (CSF) from patients with various central nervous system (CNS) diseases were determined by capillary zone electrophoresis (CZE). Under one of the two sets of conditions employed, several anionic components of CSF were separated into corresponding peaks on the electropherograms and determined. The other conditions employed were also useful in measurement of the lactate contents in CSF. The CSF levels of lactate and pyruvate and the ratios of lactate to pyruvate were elevated in patients with cerebral infarction and bacterial meningitis, whereas CSF ascorbate was reduced mainly in inflammatory disorders of the CNS. The results showed that CZE can become a powerful tool in the biochemical diagnosis of CNS diseases.
采用毛细管区带电泳（CZE）法测定了患有各种中枢神经系统（CNS）疾病患者脑脊液（CSF）中的有机酸。在所用的两组条件之一的情况下，脑脊液中的几种阴离子成分在电泳图上被分离成相应的峰并进行了测定。所用的另一组条件在测定脑脊液中的乳酸含量方面也很有用。脑梗死和细菌性脑膜炎患者脑脊液中的乳酸和丙酮酸水平以及乳酸与丙酮酸的比值升高，而脑脊液中的抗坏血酸主要在中枢神经系统炎症性疾病中降低。结果表明，毛细管区带电泳可成为中枢神经系统疾病生化诊断的有力工具。
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