Otsuka K, Karuhaka K, Higashimori M, Terabe S
Department of Industrial Chemistry, Osaka Prefectural College of Technology, Japan.
J Chromatogr A. 1994 Sep 30;680(1):317-20. doi: 10.1016/0021-9673(94)80082-0.
Optical resolution by micellar electrokinetic chromatography with N-dodecanoyl-L-serine (DSer) was investigated. Similarly to the use of sodium N-dodecanoyl-L-valinate or sodium N-dodecanoyl-L-glutamate, addition of sodium dodecyl sulfate (SDS), urea and organic modifiers such as methanol and 2-propanol (IPA) to DSer micellar solutions could give improved peak shapes and enhance the enantioselectivity. With a DSer-SDS-urea-methanol solution, six phenylthiohydantoin (PTH)DL-amino acids were separated and each enantiomeric pair was optically resolved. By using a DSer-SDS-urea-IPA solution, the same PTH-DL-amino acids were partially resolved, while PTH-DL-Thr was resolved only in this system. N-Dodecanoyl-L-aspartic acid and sodium N-tetradecanoyl-L-glutamate were also investigated, but satisfactory results were not obtained.
