Suttnar J, Fortová H, Brabec V
Department of Biochemistry, Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):119-22. doi: 10.1016/0378-4347(94)00077-8.
An ethylenedimethacrylate polymer-based matrix (EDMA 2000) is described that shortens the time for separation of haemoglobin (Hb) chains to 30 min, compared with the use of C4 large-pore Vydac columns, commonly used for reversed-phase HPLC of Hb variants. EDMA 2000 was successfully used for the isolation of an abnormal beta-chain of the unstable Hb recently characterized as Hb Nottingham.
描述了一种基于二甲基丙烯酸乙二酯聚合物的基质（EDMA 2000），与常用于血红蛋白（Hb）变体反相高效液相色谱的C4大孔Vydac柱相比，它将血红蛋白链的分离时间缩短至30分钟。EDMA 2000已成功用于分离最近鉴定为诺丁汉血红蛋白的不稳定血红蛋白的异常β链。
