Yapo Paul Angoué, Datté Jacques Y, Yapo Ayekoé, Wachman Henri
Laboratoire de Physiologie et Pharmacologie, UFR-Sciences de la Nature, Abobo-Adjamé Université, Abidjan, Côte d'Ivoire.
J Clin Lab Anal. 2004;18(2):65-9. doi: 10.1002/jcla.20007.
Rare abnormal haemoglobin (Hb) often poses a problem in the diagnosis of relative electrophoresis mobility. The rare neutral Hbs interact with the Hb S and thus can increase the severity of sickle cell anaemia. In the present study, we investigated the use of reversed-phase high-performance liquid chromatography (RP-HPLC) as a method for the identification of neutral or unstable Hb, and compared it with the well-known techniques urea-triton electrophoresis (UT) and isoelectrofocusing (IEF). The results of our experiments showed that Bucuresti Hb was separated without ambiguity by RP-HPLC. To date, no other electrophoresis techniques have been able to isolate this Hb. We also examined the Hb Nouakchott, which was the most hydrophobic Hb. Moreover, the adult and foetal chains of globins (especially the Agamma(t) and Agamma(i) chains) were quantified on the same chromatogram.
罕见异常血红蛋白（Hb）在相对电泳迁移率的诊断中常常带来问题。罕见的中性Hb与Hb S相互作用，从而可能增加镰状细胞贫血的严重程度。在本研究中，我们研究了使用反相高效液相色谱法（RP-HPLC）作为鉴定中性或不稳定Hb的方法，并将其与广为人知的尿素-曲通电泳（UT）和等电聚焦（IEF）技术进行比较。我们的实验结果表明，布加勒斯特Hb通过RP-HPLC能够被清晰分离。迄今为止，尚无其他电泳技术能够分离出这种Hb。我们还检测了最具疏水性的Hb努瓦克肖特。此外，在同一色谱图上对球蛋白的成人链和胎儿链（尤其是γA（t）和γA（i）链）进行了定量分析。
