Kasicka V, Prusík Z, Smékal O, Hlavácek J, Barth T, Weber G, Wagner H
Institute of Organic Chemistry and Biochemistry, Academy of Science of the Czech Republic, Prague.
J Chromatogr B Biomed Appl. 1994 Jun 3;656(1):99-106. doi: 10.1016/0378-4347(94)00042-5.
The use of high-performance electromigration separation methods, capillary zone electrophoresis (CZE) and capillary isotachophoresis (CITP) and continuous free-flow arrangements of these two separation principles, free-flow zone electrophoresis (FFZE) and free-flow isotachophoresis (FFITP), was investigated in the analysis and purification of the synthetic C-terminal tetrapeptide fragment (H2N-Ala-Trp-D-Phe-Lys.NH2) of the growth hormone-releasing peptide. CZE and CITP were used for microanalysis of peptide preparations after different steps of their purification. The homogeneity of the peptide preparations, including fractions of preparative separations, was quantified by relative zone length (CITP) and/or relative peak height (CZE). In addition, the data obtained by CZE and CITP (electrophoretic and electroosmotic flow migration velocities) were utilized for conversion of micro-scale capillary separations (nano- to picomole level) into the preparative separations realized by FFZE and FFITP with a capacity from tens to hundreds of milligrams per hour.
研究了高性能电迁移分离方法，即毛细管区带电泳（CZE）和毛细管等速电泳（CITP），以及这两种分离原理的连续自由流配置，即自由流区带电泳（FFZE）和自由流等速电泳（FFITP），用于生长激素释放肽合成C端四肽片段（H2N-Ala-Trp-D-Phe-Lys.NH2）的分析和纯化。CZE和CITP用于肽制剂在不同纯化步骤后的微量分析。通过相对区带长度（CITP）和/或相对峰高（CZE）对肽制剂的均匀性进行定量，包括制备分离的级分。此外，利用CZE和CITP获得的数据（电泳和电渗流迁移速度），将微量毛细管分离（纳摩尔至皮摩尔水平）转换为通过FFZE和FFITP实现的制备分离，其容量为每小时数十至数百毫克。
