Kasicka V, Prusík Z, Sázelová P, Jirácek J, Barth T
Institute of Organic Chemistry and Biochemistry, Academy of Sciences of the Czech Republic, Prague, Czech Republic.
J Chromatogr A. 1998 Feb 13;796(1):211-20. doi: 10.1016/s0021-9673(97)01114-x.
A basic theoretical description of the correlation between capillary zone electrophoresis (CZE) and free-flow zone electrophoresis (FFZE) is presented. The theory of the correlation between CZE and FFZE results from the fact that both methods are based on the same separation principle, zone electrophoresis, and both are performed in the carrierless separation medium with the same composition of the background electrolyte. The equations describing the movement of the charged and noncharged particles in the d.c. electric field applied in the capillary and in the flow-through electrophoretic chamber are presented and used for the quantitative description of the correlation between CZE and FFZE. Based on the theory of the correlation between CZE and FFZE a procedure has been developed for conversion of analytical, microscale CZE separations into continuous preparative separation processes realized by FFZE. Practical application of the developed procedure is demonstrated by CZE analysis and FFZE preparation of an octapeptide fragment of human insulin.
本文给出了毛细管区带电泳（CZE）与自由流动区带电泳（FFZE）相关性的基本理论描述。CZE与FFZE相关性的理论源于这两种方法基于相同的分离原理——区带电泳，且均在背景电解质组成相同的无载体分离介质中进行。文中给出了描述带电和不带电粒子在施加于毛细管和流通式电泳室的直流电场中运动的方程，并用于定量描述CZE与FFZE之间的相关性。基于CZE与FFZE相关性的理论，已开发出一种将分析型微尺度CZE分离转化为通过FFZE实现的连续制备分离过程的方法。通过对人胰岛素八肽片段的CZE分析和FFZE制备，展示了所开发方法的实际应用。
