Maboundou C W, Paintaud G, Bérard M, Bechtel P R
Department of Clinical Pharmacology, Jean Minjoz University Hospital, Besançon, France.
J Chromatogr B Biomed Appl. 1994 Jul 1;657(1):173-83. doi: 10.1016/0378-4347(94)80084-7.
Micellar electrokinetic capillary chromatography with sodium dodecylsulfate as anionic surfactant was used to separate simultaneously fifteen non-steroidal anti-inflammatory drugs. UV detection was performed at 254 nm. The electroosmotic flow was carefully adjusted for optimal separation. Resolution of the drugs was obtained using a buffer containing 40 mmol l-1 NaH2PO4, 0.104 mol l-1 sodium dodecylsulfate and 3% (v/v) methanol, adjusted to pH 8 with sodium hydroxide. Lack of interference was checked with a number of drugs and metabolites. Between-day coefficients of variation ranged between 2 to 10%.
以十二烷基硫酸钠作为阴离子表面活性剂的胶束电动毛细管色谱法被用于同时分离15种非甾体抗炎药。在254nm处进行紫外检测。仔细调节电渗流以实现最佳分离效果。使用含有40 mmol/L磷酸二氢钠、0.104 mol/L十二烷基硫酸钠和3%（v/v）甲醇的缓冲液，用氢氧化钠将其pH值调至8，从而实现了这些药物的分离。用多种药物和代谢物检查有无干扰。日间变异系数在2%至10%之间。
