Nielsen M S, Nielsen P V, Frisvad J C
Department of Biotechnology, Technical University of Denmark, Lyngby, Denmark.
J Chromatogr A. 1996 Jan 19;721(2):337-44. doi: 10.1016/0021-9673(95)00801-2.
Fungal metabolites were extracted from two isolates of Penicillium commune and two isolates of Aspergillus versicolor grown on yeast extract agar (YES) and Czapek yeast extract agar (CYA). Optimized conditions for analysing the extracts by high-performance capillary electrophoresis (HPCE) were obtained by experimental designs. The following nine factors were examined by a two-level fractional factorial design: concentration of the buffer ion PO4(3-) and B4O7(2-), ionic strength of buffer, buffer pH, addition of sodium dodecyl sulphate (SDS) and sodium deoxycholate (SCD), addition of acetonitrile and methanol and voltage. Four factors significantly influenced the separation of peaks in all the fungal extracts. For optimization of the HPCE method a subsequent response-surface experiment with the important factors was made with an extract from an isolate of Aspergillus versicolor. Optimum separation conditions were obtained, which gave good resolution of the components in the extract.
从在酵母提取物琼脂（YES）和察氏酵母提取物琼脂（CYA）上生长的两种普通青霉分离株和两种杂色曲霉分离株中提取真菌代谢产物。通过实验设计获得了用高效毛细管电泳（HPCE）分析提取物的优化条件。采用二级部分因子设计考察了以下九个因素：缓冲离子PO4(3-)和B4O7(2-)的浓度、缓冲液的离子强度、缓冲液pH值、十二烷基硫酸钠（SDS）和脱氧胆酸钠（SCD）的添加、乙腈和甲醇的添加以及电压。四个因素对所有真菌提取物中的峰分离有显著影响。为优化HPCE方法，用杂色曲霉分离株的提取物对重要因素进行了后续的响应面实验。获得了最佳分离条件，可以很好地分离提取物中的组分。
