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高效液相色谱法测定血浆中的抗坏血酸和尿酸

Determination of ascorbic acid and uric acid in plasma by high-performance liquid chromatography.

作者信息

Ross M A

机构信息

Rowett Research Institute, Bucksburn, Aberdeen, UK.

出版信息

J Chromatogr B Biomed Appl. 1994 Jul 1;657(1):197-200. doi: 10.1016/0378-4347(94)80087-1.

DOI:10.1016/0378-4347(94)80087-1
PMID:7952068
Abstract

A simple method with excellent reproducibility has been developed for the simultaneous determination of the plasma concentration of ascorbic acid and uric acid by reversed-phase HPLC using an ion-pairing reagent with UV detection. Coefficients of variation for the concentrations of ascorbic acid and uric acid in plasma are < 2.1% and < 3.5% respectively.

摘要

已开发出一种具有出色重现性的简单方法，用于通过使用离子对试剂和紫外检测的反相高效液相色谱法同时测定血浆中抗坏血酸和尿酸的浓度。血浆中抗坏血酸和尿酸浓度的变异系数分别<2.1%和<3.5%。

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