采用液相色谱- frit -快原子轰击质谱法分析大鼠体内(+)-4-[4-(4-甲基苯基)苯甲氧基-1-哌啶基]丁酸的尿液和胆汁代谢产物
Analysis of urinary and biliary metabolites of (+)-4-[4-(4-methylphenyl)phenylmethoxy-1-piperidinyl]butyric acid in rats by liquid chromatography-frit-fast atom bombardment mass spectrometry.
作者信息
Takahara E, Nagata O, Kato H, Ohta S, Hirobe M
机构信息
Research and Development Division, Hokuriku Seiyaku Co., Fukui, Japan.
出版信息
J Chromatogr B Biomed Appl. 1994 Aug 5;658(1):154-60. doi: 10.1016/0378-4347(94)00221-5.
The urinary and biliary metabolites of (+)-4-[4-(4-methylphenyl)phenylmethoxy-1-piperidinyl]butyric acid [(+)-MPPB] were examined in rapid-metabolizing (RM) and slow-metabolizing (SM) male Sprague-Dawley rats by means of liquid chromatography-frit-fast atom bombardment mass spectrometry. In the RM-phenotyped rats, unchanged (+)-MPPB could not be detected in urine or bile, but 4-carboxyphenyl-MPPB was detected in bile. In the SM-phenotyped rats, unchanged (+)-MPPB was detected in bile and unchanged (+)-MPPB and beta-oxidized MPPB in urine. Thus, the inter-individual difference in (+)-MPPB metabolism in rats was confirmed in vivo.
通过液相色谱- frit -快速原子轰击质谱法，在快速代谢（RM）和缓慢代谢（SM）的雄性Sprague - Dawley大鼠中检测了(+)-4-[4-(4-甲基苯基)苯甲氧基-1-哌啶基]丁酸[(+)-MPPB]的尿液和胆汁代谢物。在RM表型的大鼠中，尿液或胆汁中未检测到未变化的(+)-MPPB，但在胆汁中检测到了4-羧基苯基-MPPB。在SM表型的大鼠中，胆汁中检测到未变化的(+)-MPPB，尿液中检测到未变化的(+)-MPPB和β-氧化的MPPB。因此，在体内证实了大鼠中(+)-MPPB代谢的个体间差异。
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