Guo A L, Petraglia F, Criscuolo M, Ficarra G, Nappi R E, Palumbo M, Valentini A, Genazzani A R
Department of Endocrinology, Peking Union Medical College Hospital, Beijing P.R. China.
Eur J Endocrinol. 1994 Nov;131(5):535-9. doi: 10.1530/eje.0.1310535.
The aim of the present study was to test whether oestrous cycle is associated with the hypothalamus-pituitary-adrenal (HPA) axis function. Thus, corticosterone secretion in rats was investigated following lipopolysaccharide (LPS), acute cold-swimming or ether stress or synthetic corticotrophin-releasing factor (CRF) administration throughout the oestrous cycle. Moreover, plasma corticosterone response to cold-swimming stress or LPS administration also was studied at different times of day on pro-oestrus of di-oestrus-I. The following observations were obtained: the morning plasma corticosterone levels in control rats did not differ with the stage of the oestrous cycle; plasma corticosterone levels increased significantly following LPS administration (2 mg/kg, ip) or following acute exposure to cold (4 degrees C)-swimming or ether stress. However, this increase in plasma corticosterone levels was not related to the stage of the oestrous cycle; synthetic CRF injection induced an increase in plasma corticosterone levels constant on di-oestrus-I and pro-oestrus; plasma corticosterone response to LPS administration or acute cold-swimming stress showed diurnal changes, with the lowest values at 18.00 h, which was independent of the oestrous cycle. By showing the unchanged corticosterone response to LPS, to acute stress and to exogenous CRF throughout the oestrous cycle, and the independence of the diurnal pattern of stress response on the oestrous cycle, the present study suggests that the oestrous cycle has no influence on the HPA activity under the present experimental conditions in rats.
本研究的目的是测试发情周期是否与下丘脑-垂体-肾上腺（HPA）轴功能相关。因此，在整个发情周期中，研究了脂多糖（LPS）、急性冷游泳或乙醚应激或合成促肾上腺皮质激素释放因子（CRF）给药后大鼠皮质酮的分泌情况。此外，还在动情前期或动情间期I的不同时间研究了血浆皮质酮对冷游泳应激或LPS给药的反应。获得了以下观察结果：对照大鼠早晨血浆皮质酮水平在发情周期各阶段无差异；LPS给药（2mg/kg，腹腔注射）、急性暴露于寒冷（4℃）游泳或乙醚应激后，血浆皮质酮水平显著升高。然而，血浆皮质酮水平的这种升高与发情周期阶段无关；合成CRF注射导致动情间期I和动情前期血浆皮质酮水平持续升高；血浆皮质酮对LPS给药或急性冷游泳应激的反应呈现昼夜变化，在18:00时最低，且与发情周期无关。通过显示发情周期中对LPS、急性应激和外源性CRF的皮质酮反应不变，以及应激反应昼夜模式与发情周期无关，本研究表明在当前大鼠实验条件下，发情周期对HPA活性无影响。
