Park J C, Nemoto Y, Homma T, Sato R, Matsuoka H, Ohno H, Takatori K, Kurata H
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Microbiology (Reading). 1994 Sep;140 ( Pt 9):2409-14. doi: 10.1099/13500872-140-9-2409.
Adaptation of Aspergillus niger to short-term stress induced by three antifungal agents [amphotericin B (AMPH), miconazole (MCZ), and ketoconazole (KCZ)] was observed and evaluated quantitatively using individual hyphae. Spores were inoculated onto a poly-L-lysine-coated glass plate making up the base of a culture vessel. Potato dextrose broth (PDB) was added and the vessel incubated for 24 h at 28 degrees C. The growth rate of an arbitrarily selected test hypha was measured automatically. Exposure to AMPH (0.075 micrograms ml-1) stopped the growth of the hypha. After washing with PDB, the same concentration of AMPH was applied again. The growth of the test hypha was not inhibited. This phenomenon was defined as adaptation to the short-term stress of AMPH. Similarly, adaptation was observed with MCZ (0.01 microgram ml-1) and KCZ (0.5 microgram ml-1). The time required for the test hypha to restart growth after washing with PDB depended upon the concentration of MCZ or KCZ, but not upon the concentration of AMPH.
观察并使用单个菌丝体定量评估了黑曲霉对三种抗真菌剂（两性霉素B（AMPH）、咪康唑（MCZ）和酮康唑（KCZ））诱导的短期应激的适应性。将孢子接种到构成培养容器底部的聚-L-赖氨酸包被的玻璃板上。加入马铃薯葡萄糖肉汤（PDB），并将容器在28℃下孵育24小时。自动测量任意选择的测试菌丝的生长速率。暴露于AMPH（0.075微克/毫升）会使菌丝生长停止。用PDB洗涤后，再次施加相同浓度的AMPH。测试菌丝的生长未受到抑制。这种现象被定义为对AMPH短期应激的适应。同样，在MCZ（0.01微克/毫升）和KCZ（0.5微克/毫升）中也观察到了适应性。用PDB洗涤后测试菌丝重新开始生长所需的时间取决于MCZ或KCZ的浓度，而不取决于AMPH的浓度。
