Oh K, Matsuoka H, Sumita O, Takatori K, Kurata H
Department of Biotechnology, Faculty of Technology, Tokyo University of Agriculture and Technology, Japan.
Mycopathologia. 1992 May;118(2):71-81. doi: 10.1007/BF00442534.
Antifungal activity of several antimycotics has been evaluated using an automatic analyzing system (AAS), which is composed of a specially designed reaction vessel, microscopic observation system, image analyzing system, and computer program for automatic tracing of hypha growth. The agar plate was prepared on the ceiling of the reaction vessel, and spore mass of a fungus (Aspergillus niger) was inoculated onto it. After the preincubation at 28 degrees C for 24 h the reaction vessel was set on a microscope stage and connected to the liquid flow system. An appropriate hypha was selected for the measurement of growth process during the following steps: first contact with saline for 30 min for the adaptation, the second contact with same saline for 30 min, contact with saline containing an antimycotic substance for 60 min, and contact with flushing saline for 60 min. During a sequence of these steps, the apical tip of a growing hypha displayed on a TV monitor was followed automatically. The dynamic response of hypha to an agent was analyzed by several parameters. Morphological changes of the hypha caused by respective agents were recorded on VTR for further analysis. By using this system, the antifungal activity of antimycotics could be quantitatively determined within several hours.
使用一种自动分析系统（AAS）评估了几种抗真菌剂的抗真菌活性，该系统由一个专门设计的反应容器、显微镜观察系统、图像分析系统以及用于自动追踪菌丝生长的计算机程序组成。在反应容器的顶部制备琼脂平板，并将一种真菌（黑曲霉）的孢子团接种到上面。在28℃预孵育24小时后，将反应容器放置在显微镜载物台上并连接到液体流动系统。在接下来的步骤中选择一根合适的菌丝来测量其生长过程：首先与生理盐水接触30分钟以进行适应，第二次与相同的生理盐水接触30分钟，与含有抗真菌物质的生理盐水接触60分钟，以及与冲洗生理盐水接触60分钟。在这些步骤的序列中，在电视监视器上显示的生长菌丝的顶端会被自动追踪。通过几个参数分析菌丝对一种药剂的动态反应。由各自药剂引起的菌丝形态变化被记录在录像带上以供进一步分析。通过使用该系统，可以在几个小时内定量测定抗真菌剂的抗真菌活性。
