Wahle P, Lübke J, Naegele J R
Lehrstuhl Allgemeine Zoologie und Neurobiologie, Ruhr-Universität, Bochum, Germany.
Eur J Neurosci. 1994 Jul 1;6(7):1167-78. doi: 10.1111/j.1460-9568.1994.tb00615.x.
During development, the subplate zone of the cat neocortex contains neuronal populations with distinct morphological and neurochemical phenotypes. A subset of those are specifically recognized by a mouse monoclonal antibody termed SUBPLATE-1 (SP1), which was generated against tissue homogenates of kitten cortical white matter. SP1 stains cell bodies and proximal dendrites, but rarely distal dendrites, axonal arbors or spines. In order to characterize morphologically the SP1 immunoreactive subplate cell types, we combined SP1 immunohistochemistry with intracellular iontophoretic injections of Lucifer yellow. The majority of double-labelled neurons were inverted pyramids with a single thicker spine-covered dendrite that descended into the white matter and a tuft of thinner spinous dendrites that ascended from the upper somatic pole, but generally remained confined to the white matter. Other double-labelled neurons were multipolar to bitufted, although often equipped with one thicker descending dendrite. In inverted pyramidal cells, the axons originated from the descending dendrite or, more rarely, from the lower portion of the soma, and descended into the white matter. They formed collaterals recurring toward the grey matter. The presence of dendritic spines on double-labelled pyramidal cells and the axonal arborization patterns were two novel features not revealed previously by SP1 immunohistochemistry alone. The inverted pyramidal morphology was typical for double-labelled neurons located in the subplate zone below the apices of the gyri, whereas those located below the flanks or sulci or deep in the white matter often displayed a bitufted or multipolar spinous morphology. A minority of the double-labelled neurons were multipolar with smooth dendrites and locally branching axons. These results suggest that in the cat subplate zone, a majority of the cells expressing the SP1 antigen are spinous, and we termed the spinous subplate cells 'subplate pyramidal neurons'.
在发育过程中，猫新皮质的亚板层区域包含具有不同形态和神经化学表型的神经元群体。其中一部分神经元可被一种名为“亚板层 - 1（SP1）”的小鼠单克隆抗体特异性识别，该抗体是针对小猫皮质白质的组织匀浆产生的。SP1可标记细胞体和近端树突，但很少标记远端树突、轴突分支或棘突。为了从形态学上表征SP1免疫反应性亚板层细胞类型，我们将SP1免疫组织化学与荧光黄的细胞内离子电渗注入相结合。大多数双标记神经元是倒置的锥体神经元，有一条较粗的、覆盖着棘突的树突向下延伸至白质，还有一束较细的棘突状树突从胞体上极向上延伸，但通常仍局限于白质内。其他双标记神经元是多极到双簇状的，不过通常有一条较粗的向下树突。在倒置锥体神经元中，轴突起源于向下的树突，或者更少见地起源于胞体下部，然后向下进入白质。它们形成向灰质折返的侧支。双标记锥体神经元上树突棘的存在以及轴突分支模式是仅靠SP1免疫组织化学之前未揭示的两个新特征。倒置锥体形态对于位于脑回顶端下方亚板层区域的双标记神经元来说是典型的，而位于脑回侧面或脑沟下方或白质深处的那些神经元通常呈现双簇状或多极棘突形态。少数双标记神经元是多极的，有光滑的树突和局部分支的轴突。这些结果表明，在猫的亚板层区域，大多数表达SP1抗原的细胞是有棘突的，我们将有棘突的亚板层细胞称为“亚板层锥体神经元”。
