Lee Y H, Shin D W
Department of Parasitology, College of Medicine, Chungnam National University, Taejon, Korea.
Korean J Parasitol. 1994 Sep;32(3):185-93. doi: 10.3347/kjp.1994.32.3.185.
The present study was undertaken to assess the role of cytokines in the activation of peritoneal macrophages from Toxoplasma-infected mice. Peritoneal macrophages from Toxoplasma-infected mice (10 cysts of Beverley strain/mouse) were harvested 8 weeks after infection, and incubated with the mitogen-induced lymphokine, recombinant mouse interferon-gamma (IFN-gamma), recombinant mouse tumor necrosis factor-alpha (TNF-alpha) alone or in combination with IFN-gamma (IFN-gamma/TNF-alpha) for 24 hr at 37 degrees C, 5% CO2. Macrophage activation was measured by the amount of H2O2 and NO2- production, and anti-Toxoplasma activities of macrophages. IFN-gamma or IFN-gamma/TNF-alpha-treated macrophages from Toxoplasma-infected mice revealed significantly higher H2O2 production than resident macrophages from Toxoplasma-infected mice. The production of NO2- by TNF-alpha-, IFN-gamma- or IFN-gamma/TNF-alpha-treated macrophages from Toxoplasma-infected mice were significantly higher than that by resident macrophages, whereas lymphokine-treated group produced similar amount as that produced by resident macrophages. Anti-Toxoplasma activities of cytokine-treated macrophages from Toxoplasma-infected mice were significantly higher than those of resident macrophages. IFN-gamma-treated macrophages were significantly increased production of H2O2 and NO2-, and anti-Toxoplasma activities of macrophages between normal and Toxoplasma-infected mice, whereas the other cytokine-treated groups were not significant differences between them. These data suggested that IFN-gamma was the only one of cytokines capable of significantly activating the peritoneal macrophages from Toxoplasma-infected mice.
本研究旨在评估细胞因子在弓形虫感染小鼠腹腔巨噬细胞激活中的作用。在感染8周后，收集来自弓形虫感染小鼠（每只小鼠感染10个贝弗利株包囊）的腹腔巨噬细胞，并在37℃、5%二氧化碳条件下，将其与丝裂原诱导的淋巴因子、重组小鼠干扰素-γ（IFN-γ）、重组小鼠肿瘤坏死因子-α（TNF-α）单独或与IFN-γ联合（IFN-γ/TNF-α）孵育24小时。通过过氧化氢和亚硝酸根的产生量以及巨噬细胞的抗弓形虫活性来测定巨噬细胞的激活情况。来自弓形虫感染小鼠经IFN-γ或IFN-γ/TNF-α处理的巨噬细胞产生的过氧化氢量显著高于来自弓形虫感染小鼠的驻留巨噬细胞。来自弓形虫感染小鼠经TNF-α、IFN-γ或IFN-γ/TNF-α处理的巨噬细胞产生的亚硝酸根显著高于驻留巨噬细胞，而经淋巴因子处理的组产生的量与驻留巨噬细胞产生的量相似。来自弓形虫感染小鼠经细胞因子处理的巨噬细胞的抗弓形虫活性显著高于驻留巨噬细胞。IFN-γ处理的巨噬细胞显著增加了过氧化氢和亚硝酸根的产生，以及正常小鼠和弓形虫感染小鼠巨噬细胞的抗弓形虫活性，而其他细胞因子处理组之间没有显著差异。这些数据表明，IFN-γ是唯一能够显著激活来自弓形虫感染小鼠腹腔巨噬细胞的细胞因子。
