Terashima T, Ochiishi T, Yamauchi T
Department of Anatomy, Tokyo Metropolitan Institute for Neuroscience, Japan.
Brain Res. 1994 Jul 4;650(1):133-9. doi: 10.1016/0006-8993(94)90215-1.
To determine whether or not calcium/calmodulin-dependent protein kinase II (CaM kinase II) is localized in the ganglion cells in the rat retina, we labeled ganglion cells by injection of Fast blue (FB) into the lateral geniculate nucleus and then stained the retina immunohistochemically with monoclonal antibodies which react specifically with the alpha and beta isoforms of CaM kinase II. Eighty and 90% of the FB-labeled ganglion cells in the ganglion cell layer were immunoreactive with the alpha and beta antibodies, respectively, suggesting that both alpha and beta isoforms of CaM kinase II are expressed in most ganglion cells which project to the lateral geniculate nucleus.
为了确定钙/钙调蛋白依赖性蛋白激酶II（CaM激酶II）是否定位于大鼠视网膜的神经节细胞中，我们通过向外侧膝状核注射固蓝（FB）来标记神经节细胞，然后用与CaM激酶II的α和β亚型特异性反应的单克隆抗体对视网膜进行免疫组织化学染色。神经节细胞层中80%和90%的FB标记神经节细胞分别与α和β抗体发生免疫反应，这表明CaM激酶II的α和β亚型在大多数投射到外侧膝状核的神经节细胞中均有表达。
