无乳链球菌（B 组）中的 R 质粒
R plasmids in Streptococcus agalactiae (group B).
作者信息
Horodniceanu T, Bouanchaud D H, Bieth G, Chabbert Y A
出版信息
Antimicrob Agents Chemother. 1976 Nov;10(5):795-801. doi: 10.1128/AAC.10.5.795.
Abstract
Two plasmids determining resistance to tetracycline (RIP500) and to chloramphenicol, erythromycin, lincomycin, and pristinamycin I (RIP501) were isolated from a strain of Streptococcus agalactiae. The frequency-of-resistance loss is very low for RIP500 (<3 x 10(4)) but higher for RIP501 (the efficiency was dependent upon the curing agents and incubation temperature and varied between 0.5 and 96%). Derivatives susceptible to all drugs were also obtained. RIP500 and RIP501 have similar molecular weights (17.9 x 10(6) and 20 x 10(6), respectively) and represent different percentages of total deoxyribonucleic acid (0.4 and 4%, respectively). The number of copies of RIP500 and RIP501 per cell is different, and these plasmids are likely replicated under different kinds of control (stringent and/or relaxed). No plasmid deoxyribonucleic acid was found in a derivative of strain B96 susceptible to all drugs.
摘要
从无乳链球菌的一个菌株中分离出了两种分别决定对四环素（RIP500）和氯霉素、红霉素、林可霉素及原始霉素I（RIP501）耐药性的质粒。RIP500的耐药性丧失频率很低（<3×10⁻⁴），但RIP501的耐药性丧失频率较高（其效率取决于消除剂和培养温度，在0.5%至96%之间变化）。还获得了对所有药物敏感的衍生物。RIP500和RIP501具有相似的分子量（分别为17.9×10⁶和20×10⁶），分别占总脱氧核糖核酸的不同比例（分别为0.4%和4%）。每个细胞中RIP500和RIP501的拷贝数不同，并且这些质粒可能在不同类型的控制（严谨型和/或松弛型）下进行复制。在对所有药物敏感的B96菌株的一个衍生物中未发现质粒脱氧核糖核酸。
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