Desiderio M A, Limonta D M, Bardella L
Institute of General Pathology, University of Milan, Italy.
Cancer Lett. 1994 Nov 25;87(1):33-8. doi: 10.1016/0304-3835(94)90406-5.
The expression of the two proto-oncogenes ornithine decarboxylase and c-met was examined during various phases of growth of Yoshida AH-130 ascites hepatoma. Ornithine decarboxylase (ODC) and c-met mRNA levels declined progressively from day 5 (exponential growth-phase) until day 14 (quasi-stationary growth-phase). Transcription rate for both the genes remained constant between days 5 and 10, while decreasing at day 14. ODC activity was consistent with ODC mRNA level during hepatoma growth. In host liver, ODC mRNA accumulated 5 and 14 days after tumor transplantation, while c-met mRNA level was elevated until day 10 and diminished at day 14. ODC activity triplicated at day 14 in host liver. The progressive decline in the expression of ODC and c-met observed in hepatoma might be one of the mechanisms important for the control of tumor growth.
在吉田AH - 130腹水肝癌生长的各个阶段，检测了两种原癌基因鸟氨酸脱羧酶和c - met的表达情况。从第5天（指数生长期）到第14天（准静止生长期），鸟氨酸脱羧酶（ODC）和c - met mRNA水平逐渐下降。在第5天至第10天期间，这两个基因的转录率保持恒定，而在第14天下降。在肝癌生长过程中，ODC活性与ODC mRNA水平一致。在宿主肝脏中，肿瘤移植后第5天和第14天ODC mRNA积累，而c - met mRNA水平在第10天之前升高，在第14天降低。宿主肝脏中ODC活性在第14天增加了两倍。在肝癌中观察到的ODC和c - met表达的逐渐下降可能是控制肿瘤生长的重要机制之一。
