Chen Y, Mei Q, Liu Y, Wang Q
Research Institute of Infectious and Parasitic Diseases, Chongqing University of Medical Sciences.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(2):115-8.
Rat Pneumocystis carinii cyst soluble antigen (CSA) was analysed by SDS-PAGE, and more than 20 polypeptide bands were found. The molecular weights of the major bands were > 100kDa, 85-94kDa, 67kDa, 52kDa and 34kDa, respectively. The specific IgG antibodies in the sera from healthy blood donors recognizing the 110kDa, 105kDa, 85kDa, 67kDa, 52kDa and 46kDa polypeptide antigens were identified by immunoblot analysis. The total positive rate of IgG specific antibodies was 56.7% (59/104). The 85kDa polypeptide antigen could also react with the monoclonal antibody derived from human Pneumocystis carinii. The positive rate of the specific anti-85kDa-IgG antibody was 37.5% (39/104), but the specific anti-Pneumocystis carinii IgM antibodies were not detected in all the sera examined. Three of 7 serum samples from the patients with Pneumocystis carinii pneumonia were positive for specific anti-85kDa-IgG antibody (Figs 1-4).
用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）分析大鼠卡氏肺孢子虫包囊可溶性抗原（CSA），发现20多条多肽带。主要条带的分子量分别>100kDa、85 - 94kDa、67kDa、52kDa和34kDa。通过免疫印迹分析鉴定了健康献血者血清中识别110kDa、105kDa、85kDa、67kDa、52kDa和46kDa多肽抗原的特异性IgG抗体。IgG特异性抗体的总阳性率为56.7%（59/104）。85kDa多肽抗原也能与人源卡氏肺孢子虫单克隆抗体发生反应。特异性抗85kDa-IgG抗体的阳性率为37.5%（39/104），但在所检测的所有血清中均未检测到特异性抗卡氏肺孢子虫IgM抗体。7例卡氏肺孢子虫肺炎患者的血清样本中有3例特异性抗85kDa-IgG抗体呈阳性（图1 - 4）。
