Xu D, Lou W
Department of Parasitology, Shanghai Second Medical University.
Zhongguo Ji Sheng Chong Xue Yu Ji Sheng Chong Bing Za Zhi. 1994;12(2):138-9.
Serum samples were collected from normal rabbits, normal persons, Schistosoma japonicum-infected rabbits and human cases respectively, and were treated by TCA precipitation and PEG precipitation, and subsequently detected by dot-ELISA. The results showed that both the supernatants of TCA-treated antisera from infected human cases and rabbits and the precipitants of PEG-treated antisera of the same origins showed positive reactions, while no positive reactions were found for sera from normal rabbits and normal persons. It was exhibited that dot-ELISA detected not only the circulating antigen of schistosomes but also the immunocomplex.
分别从正常兔、正常人、日本血吸虫感染兔和人类病例中采集血清样本，经三氯乙酸沉淀和聚乙二醇沉淀处理，随后采用斑点酶联免疫吸附测定法进行检测。结果显示，感染人类病例和兔的三氯乙酸处理抗血清的上清液以及相同来源的聚乙二醇处理抗血清的沉淀物均呈阳性反应，而正常兔和正常人的血清未发现阳性反应。结果表明，斑点酶联免疫吸附测定法不仅能检测血吸虫循环抗原，还能检测免疫复合物。
