Grønlund B, Grønlund L, Christensen N J
Department of Internal Medicine and Endocrinology, Herlev Hospital, University of Copenhagen, Denmark.
Clin Physiol. 1994 Jul;14(4):467-74. doi: 10.1111/j.1475-097x.1994.tb00405.x.
Changes in local noradrenaline level in subcutaneous tissue were measured in four healthy males (23-33 years) during cold stimulation using a microdialysis technique. The subcutaneous dialysate noradrenaline concentration increased during cold exposure by a factor of 3.5 (P < 0.003). A correction factor due to plasma overflow of noradrenaline to subcutaneous tissue was calculated from noradrenaline infusion studies. This correction had a minimal effect on the observed increase in dialysate noradrenaline concentration and the level of significance was only slightly altered after correction for plasma overflow. The microdialysis technique in combination with a sensitive radioenzymatic noradrenaline assay may be useful to assess changes in local subcutaneous noradrenaline level in man during physiological stimulation.
采用微透析技术，对4名健康男性（23 - 33岁）在冷刺激期间皮下组织中去甲肾上腺素水平的变化进行了测量。在冷暴露期间，皮下透析液中去甲肾上腺素浓度增加了3.5倍（P < 0.003）。根据去甲肾上腺素输注研究计算了由于血浆中去甲肾上腺素溢入皮下组织所致的校正因子。该校正对所观察到的透析液中去甲肾上腺素浓度的增加影响极小，校正血浆溢入后显著性水平仅略有改变。微透析技术与灵敏的放射酶法去甲肾上腺素测定相结合，可能有助于评估人体在生理刺激期间皮下局部去甲肾上腺素水平的变化。
