Bale M J, Jones R N, Erwin M E
University of Iowa College of Medicine, Iowa City 52242.
Diagn Microbiol Infect Dis. 1994 May;19(1):65-8. doi: 10.1016/0732-8893(94)90054-x.
Several multilaboratory collaborative studies using broth microdilution and agar dilution (gonococcal tests only) methods of susceptibility testing were performed to establish quality-control (QC) giudelines. Replicate dilution tests with multiple lots of media were performed with National Committee for Clinical Laboratory Standards recommended QC strains (Escherichia coli ATCC 25922, Pseudomonas aeruginosa ATCC 27853, Enterococcus faecalis ATCC 29212, and Staphylococcus aureus ATCC 29213) by using the following antimicrobials: biapenem, DU-6859a, FK-037, levofloxacin, and grepafloxacin. In addition QC MIC ranges, using appropriate medium modification for Haemophilus influenzae ATCC 49247 and Neisseria gonorrhoeae ATCC 49226 were reported on four (DU-6859a, FK-037, levofloxacin, and grepafloxacin) and two (ceftizoxime and FK-037) antimicrobials, respectively.
开展了多项多实验室协作研究,采用肉汤微量稀释法和琼脂稀释法(仅用于淋球菌检测)进行药敏试验,以制定质量控制(QC)指南。使用国家临床实验室标准委员会推荐的QC菌株(大肠埃希菌ATCC 25922、铜绿假单胞菌ATCC 27853、粪肠球菌ATCC 29212和金黄色葡萄球菌ATCC 29213),对多批培养基进行重复稀释试验,所使用的抗菌药物如下:比阿培南、DU-6859a、FK-037、左氧氟沙星和格帕沙星。此外,还分别报告了针对4种抗菌药物(DU-6859a、FK-037、左氧氟沙星和格帕沙星)以及2种抗菌药物(头孢唑肟和FK-037)的QC MIC范围,其中针对流感嗜血杆菌ATCC 49247使用了适当的培养基改良方法,针对淋病奈瑟菌ATCC 49226则采用了相应方法。