Castro J I, Romano M C
Department of Physiology, Biophysics & Neurosciences, Center of Investigation & Advanced Studies of the IPN, Mexico, D.F. Mexico.
Domest Anim Endocrinol. 1994 Jul;11(3):261-9. doi: 10.1016/0739-7240(94)90017-5.
This report describes the influences of fetal serum and gonadotropins on the development and function of chick testicular cells in a culture system that allows long term control of culture conditions. Testis of new born male chicks were dissociated and cultured in different conditions and the production of testosterone measured by RIA. 1. To investigate the effect of serum the cells were wither cultured in a defined medium composed by Dulbecco's modified medium (DMM) plus 0.1% albumin or media containing DMM plus 10% fetal bovine serum (FBS). The presence of serum increased protein content of the cultures, but no differences were found in basal testosterone production. When hCG (2IU/ml) was added to both culture media, the increment in testosterone production hence produced, was greater when cells were cultured in defined medium. 2. Cells were precultured for 24 hr in either defined serum media with or without hCG. The media were then replaced with DMM plus BSA and 1-methyl 3-isobutyl xanthine (IBMX) in the presence or absence of hCG and cells further incubated for 2 hr. Addition of hCG increased testosterone production by cells cultured in defined medium and enhanced its subsequent response to hCG stimulation. Cells cultured in serum medium maintained their ability to respond to hCG, but the addition of this hormone to the medium, abolished the response to further hCG-stimulation. 3. hCG caused a dose-related increased in testosterone production by cells cultured in defined and serum containing media, but concentrations of hCG above 2 IU/ml depressed testosterone production in the latter group.(ABSTRACT TRUNCATED AT 250 WORDS)
本报告描述了在一个能够长期控制培养条件的培养系统中，胎牛血清和促性腺激素对鸡睾丸细胞发育和功能的影响。将新生雄性雏鸡的睾丸解离并在不同条件下培养，通过放射免疫分析法测定睾酮的产生。1. 为研究血清的作用，细胞分别在由杜尔贝科改良培养基（DMM）加0.1%白蛋白组成的限定培养基中培养，或在含有DMM加10%胎牛血清（FBS）的培养基中培养。血清的存在增加了培养物中的蛋白质含量，但基础睾酮产生量未发现差异。当向两种培养基中加入人绒毛膜促性腺激素（hCG，2IU/ml）时，在限定培养基中培养的细胞产生的睾酮增量更大。2. 细胞在含或不含hCG的限定血清培养基中预培养24小时。然后在有或无hCG的情况下，将培养基换成DMM加牛血清白蛋白（BSA）和1-甲基-3-异丁基黄嘌呤（IBMX），细胞再孵育2小时。加入hCG增加了在限定培养基中培养的细胞的睾酮产生，并增强了其随后对hCG刺激的反应。在血清培养基中培养的细胞保持了对hCG的反应能力，但向培养基中加入该激素后，消除了对进一步hCG刺激的反应。3. hCG使在限定培养基和含血清培养基中培养的细胞的睾酮产生呈剂量相关增加，但hCG浓度高于2IU/ml时，会抑制后一组中的睾酮产生。（摘要截短至250字）
