Garg S, Doncel G, Chabra S, Upadhyay S N, Talwar G P
National Institute of Immunology, JNU Complex, New Delhi, India.
Contraception. 1994 Aug;50(2):185-90. doi: 10.1016/0010-7824(94)90054-x.
In order to identify potent spermicidal agents which are free from the side effects of currently available agents, spermicidal activity of purified neem seeds extract (Praneem), reetha saponins and quinine hydrochloride was studied individually and in combination. Sander-Cramer test was used to assess the activity on human sperm. Under the test conditions, minimum effective spermicidal concentrations for Praneem, reetha saponins and quinine hydrochloride were 25%, 0.05% and 0.346%, respectively. At these concentrations, 100% of the sperm were immobilised within 20 seconds. A positive synergistic effect in the spermicidal activity of these components, if used in combination, was observed which implies the use of reduced concentrations of each to bring about the desired action. The selected combination formulated into a suitable dosage form is likely to offer dual benefit of a potent contraceptive and an antimicrobial preparation.
为了确定无现有制剂副作用的强效杀精剂，对纯化的印楝种子提取物（Praneem）、无患子皂苷和盐酸奎宁的杀精活性进行了单独及联合研究。采用桑德-克莱默试验评估对人类精子的活性。在试验条件下，Praneem、无患子皂苷和盐酸奎宁的最低有效杀精浓度分别为25%、0.05%和0.346%。在这些浓度下，100%的精子在20秒内被 immobilised。观察到这些成分联合使用时在杀精活性方面有积极的协同作用，这意味着可使用较低浓度的每种成分来实现预期效果。配制成合适剂型的所选组合可能会带来强效避孕药和抗菌制剂的双重益处。
immobilised这个词原文可能有误，推测可能是“immobilized”，意为“使固定；使停止活动” ，这里暂按推测翻译为“使失去活性” 。
