Sharma S K, SaiRam M, Ilavazhagan G, Devendra K, Shivaji S S, Selvamurthy W
Defense Institute of Physiology and Allied Sciences, Timarpur, Delhi, India.
Contraception. 1996 Dec;54(6):373-8. doi: 10.1016/s0010-7824(96)00204-1.
The present study was undertaken to elucidate the mechanism of spermicidal action of NIM-76, a fraction isolated from neem oil. The spermicidal activity of NIM-76 was confirmed using a fluorescent staining technique. NIM-76 was found to affect the motility of the sperm in a dose-dependent manner. Supplementation of pentoxifylline, which is known to enhance the motility of the sperm, could not prevent the spermicidal action of NIM-76. There was a gradual leakage of cytosolic LDH from the sperm in the presence of NIM-76. Electron microscopic studies revealed the formation of pores and vesicles over the sperm head, indicating the damage to the cell membrane. Membrane fluidization studies did not reveal any significant change in the fluidity of sperm cell membrane structure.
本研究旨在阐明从印楝油中分离出的组分NIM-76的杀精作用机制。采用荧光染色技术证实了NIM-76的杀精活性。发现NIM-76以剂量依赖的方式影响精子的活力。补充已知可增强精子活力的己酮可可碱并不能阻止NIM-76的杀精作用。在存在NIM-76的情况下，精子胞质乳酸脱氢酶逐渐泄漏。电子显微镜研究显示精子头部形成孔和囊泡，表明细胞膜受到损伤。膜流动性研究未发现精子细胞膜结构的流动性有任何显著变化。
