Chen S Z, Han J S
Neuroscience Research Center, Beijing Medical University.
Chin Med J (Engl). 1994 Jun;107(6):440-3.
In the present study, the radioreceptor binding method was used to determine the changes of IP3 content in rat brain and dorsal spinal cord of high frequency (100 Hz) electroacupuncture (EA) analgesia and EA tolerance rat. The control levels of IP3 in rat brain (less cerebellum and cortex) and dorsal spinal cord were 6.3 +/- 0.78 pmol/mg protein and 3.4 +/- 0.60 pmol/mg protein, respectively. The results showed that IP3 in brain increased gradually within 45 min after stimulation in EA analgesia rat. Meanwhile, the dorsal spinal cord IP3 content decreased significantly 15 min, 30 min after EA stimulation and recovered to control level 45 min after EA stimulation. In EA tolerance rat, IP3 contents markedly increased in brain. And IP3 content in the spinal cord also increased dramatically within 30 min, but decreased rapidly to control level 45 min after EA stimulation. The IP3 level in EA tolerance rat brain and spinal cord was much higher than that in EA analgesia rat (P < 0.01). The studies first reported that high frequency (100 Hz) EA may be linked to PI system in its signal transduction pathways.
在本研究中，采用放射受体结合法测定高频（100Hz）电针镇痛及电针耐受大鼠脑和脊髓背角中肌醇三磷酸（IP3）含量的变化。大鼠脑（小脑和皮质除外）和脊髓背角中IP3的对照水平分别为6.3±0.78pmol/mg蛋白和3.4±0.60pmol/mg蛋白。结果显示，电针镇痛大鼠在刺激后45分钟内脑内IP3逐渐增加。同时，电针刺激后15分钟、30分钟脊髓背角IP3含量显著降低，电针刺激后45分钟恢复至对照水平。在电针耐受大鼠中，脑内IP3含量明显增加。脊髓中IP3含量在30分钟内也急剧增加，但在电针刺激后45分钟迅速降至对照水平。电针耐受大鼠脑和脊髓中的IP3水平远高于电针镇痛大鼠（P<0.01）。本研究首次报道高频（100Hz）电针在其信号转导途径中可能与磷脂酰肌醇系统有关。
