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[两种不同草种叶片中硅定位的比较电子显微镜研究]

[Comparative electron microscopic studies on the localization of silicon in leaves of two different grass species].

作者信息

Bode E, Kozik S, Kunz U, Lehmann H

机构信息

Botanischen Institut, Tierärztlichen Hochschule Hannover.

出版信息

Dtsch Tierarztl Wochenschr. 1994 Sep;101(9):367-72.

PMID:7956815
Abstract

The quality of green fodder can be diminished by lignifying and mineralizing the plant cell walls. In many grasses epidermal cell walls are penetrated with silica; those plants are extremely rough and sharp-edged and are not ingested by animals. The process of silicification of cell walls was studied comparatively in two grasses, the soft Perennial Ryegrass (Lolium perenne) and the rough Tufted Hair-grass (Deschampsia caespitosa). In Lolium only the epidermal cell walls of the leaf edges and the trichomes are penetrated with silica, whereas in Deschampsia silica could be demonstrated in the walls of all epidermal cells and of the trichomes. In addition, silica containing cells, the so called silica bodies, were found in Deschampsia leaves. Silica was detected using two analytical electron-microscopical techniques, the Electron-Energy-Loss-Spectroscopy (EELS) and the Electron-Spectroscopic-Imaging (ESI).

摘要

植物细胞壁的木质化和矿化会降低青饲料的质量。在许多禾本科植物中,表皮细胞壁含有硅;这些植物极其粗糙且边缘锋利,动物不会食用。对两种禾本科植物——柔软的多年生黑麦草(Lolium perenne)和粗糙的丛生发草(Deschampsia caespitosa)——细胞壁的硅化过程进行了比较研究。在黑麦草中,只有叶缘和毛状体的表皮细胞壁含有硅,而在丛生发草中,所有表皮细胞和毛状体的细胞壁都能检测到硅。此外,在丛生发草的叶片中发现了含硅细胞,即所谓的硅质体。使用两种分析电子显微镜技术——电子能量损失谱(EELS)和电子光谱成像(ESI)——检测到了硅。

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