Instituto de Geología de Costas y del Cuaternario, Facultad de Ciencias Exactas y Naturales, Universidad Nacional de Mar del Plata, Mar del Plata, Argentina.
Plant Biol (Stuttg). 2012 Jul;14(4):596-604. doi: 10.1111/j.1438-8677.2011.00530.x. Epub 2011 Dec 20.
Many studies relate silica content in plants with internal or external factors; however, few works analyse the effect of these factors on the silicification of different cell types. In this study, we examined the effect of leaf section and leaf position, and environmental conditions on the percentages of silicified epidermal cells of a native Pampean panicoid grass, Bothriochloa laguroides D. C. Pilger. Two different environmental situations were selected for the collection of plants: a natural wetland and a quartzite quarry, located in the southeast Buenos Aires province, Argentina. Clarification and staining methodologies were applied so as to study the distribution of silicified cells in different sections of leaves of the plants collected. Two and three-factor anovas were applied to the data. Between 13% and 19% of total cells of the adaxial epidermis of leaf blades were silicified. Typical silica short cells were the largest contributor to total silicified cells (53-98%), while the second largest contributor was bulliform cells (0-30%). Percentages of total silicified cells were higher in superior than in inferior leaves, while values from leaf sections varied. When collection sites were compared, plants growing in Los Padres pond, where the silica content in soils is higher, had the higher percentage of silicified cells. Among all types of cell, bulliform cells showed differences in the proportion of silicified cells between leaf position and section and collection site. These results show that silica availability in soils is an important factor that conditions silica accumulation and overlaps with the transpiration effect.
许多研究都将植物中的硅含量与内部或外部因素联系起来；然而，很少有研究分析这些因素对不同细胞类型硅化的影响。在这项研究中，我们研究了叶片切片和叶片位置以及环境条件对本地潘帕斯恐慌草（Bothriochloa laguroides D. C. Pilger）表皮硅化细胞百分比的影响。为了收集植物，我们选择了两种不同的环境情况：一个自然湿地和一个位于阿根廷布宜诺斯艾利斯省东南部的石英矿。应用了澄清和染色方法来研究收集到的植物叶片不同部位硅化细胞的分布。对数据进行了两因素和三因素方差分析。叶片叶片腹表皮的总细胞中，有 13%-19%被硅化。典型的硅短细胞是总硅化细胞的最大贡献者（53%-98%），而第二大贡献者是泡状细胞（0%-30%）。上叶的总硅化细胞百分比高于下叶，而叶片切片的数值则有所不同。当比较采集地点时，生长在 Los Padres 池塘的植物，其土壤中的硅含量较高，具有更高比例的硅化细胞。在所有类型的细胞中，泡状细胞在叶片位置、切片和采集地点之间的硅化细胞比例上存在差异。这些结果表明，土壤中硅的可用性是影响硅积累的重要因素，与蒸腾作用的影响重叠。
