Tanaka I
Nihon Yakurigaku Zasshi. 1976 Sep;72(6):673-87.
Albino rats of Wistar strain (Tamura 1950) breeded in a closed colony were administered arsenic trioxide orally during pregnancy (from the 0 day to the 20th day). Organs of fetuses and mother rats were exenterated on the 21st day of gestation and the contents of arsenic measured using an arsenic analyzer unit with atomic absorption spectrophotometry. Whole organs of the fetus were separated into 3 groupings i.e. liver, brain and remaining organs. The contents of arsenic in the organs in each of these groupings and in the placenta were measured. Even in the non-administered group, arsenic was detected in the every organ. In the arsenic administered group, the content of arsenic in the placenta was the highest among the four preparations tested; and the content in the liver and remaining organs was considerably high, but was low in the brain. The level of accumulation of arsenic differed between each organ. In the placenta, the accumulation reached a plateau, and in the brain this accumulation was below one-tenth that in the liver. In the non-administered group, arsenic was detected in the liver, kidney, spleen and brain of mother rats. In the group on arsenite, the content in the kidney and spleen was large, followed by a large amount in the liver and in the brain respectively. The level of accumulation of arsenic in mother rats differed between each organ. Arsenite was administered with antidotes such as dimercaprol, thioctic acid and L-ascorbic acid during pregnancy (from the 0 day to the 5th day). In this group the content of arsenic in the remaining organs was statistically less than that of the control group. The content in the brain was slightly reduced by a co-administration of the antidotes, however, there was no statistical difference in the placenta and liver between the antidote-treated and control groups. The content of arsenic in the kidney of mother rats treated with antidotes was statistically less than that of the controls. Whether or not the content of arsenic in organs of fetuses and mother rats was affected by a milk diet was also studied. The content of arsenic in the organs of fetuses showed no statistical difference between the animals on an Oriental stock diet group and those on the milk diet. On the other hand, the content of arsenic in the kidney of mother rats on the milk diet was statistically less than seen in those in the Oriental stock diet group.
在封闭群体中繁殖的Wistar品系白化大鼠（田村，1950）在孕期（从第0天至第20天）经口给予三氧化二砷。在妊娠第21天取出胎儿和母鼠的器官，使用带原子吸收分光光度法的砷分析仪测定砷含量。胎儿的整个器官分为3组，即肝脏、大脑和其余器官。测定这些组中每个器官以及胎盘中的砷含量。即使在未给药组中，每个器官也都检测到了砷。在砷给药组中，胎盘中的砷含量在所测试的四种制剂中最高；肝脏和其余器官中的含量相当高，但大脑中的含量较低。砷在各器官中的蓄积水平不同。在胎盘中，蓄积达到平台期，而在大脑中，这种蓄积低于肝脏中的十分之一。在未给药组中，在母鼠的肝脏、肾脏、脾脏和大脑中检测到了砷。在亚砷酸盐组中，肾脏和脾脏中的含量较高，肝脏和大脑中的含量分别次之。砷在母鼠各器官中的蓄积水平不同。在孕期（从第0天至第5天）给亚砷酸盐组同时给予解毒剂，如二巯丙醇、硫辛酸和L-抗坏血酸。在该组中，其余器官中的砷含量在统计学上低于对照组。解毒剂联合给药使大脑中的含量略有降低，然而，解毒剂治疗组和对照组在胎盘和肝脏中的含量没有统计学差异。用解毒剂治疗的母鼠肾脏中的砷含量在统计学上低于对照组。还研究了胎儿和母鼠器官中的砷含量是否受乳类饮食的影响。东方种源饮食组和乳类饮食组动物胎儿器官中的砷含量没有统计学差异。另一方面，乳类饮食组母鼠肾脏中的砷含量在统计学上低于东方种源饮食组母鼠。
