Kimata H
Department of Pediatrics, Kyoto University Hospital, Japan.
Eur J Immunol. 1994 Nov;24(11):2910-3. doi: 10.1002/eji.1830241149.
The effects of gangliosides on human plasma cell responses were studied. Among the various gangliosides tested, only GM1 enhanced immunoglobulin (Ig) production and proliferation in the human plasma cell lines, IM-9 and AF-10, while other gangliosides (GM2, GM3, GD1a, GD1b, GD3, GT1b, and GQ1b) had no effect. Among the various cytokines tested, including interleukin (IL)-1 beta, IL-2, IL-3, IL-4, IL-5, IL-6, IL-9, IL-10, IL-12, IL-13, interferon (IFN)-alpha and IFN-gamma, only IL-6 enhanced Ig production and proliferation in IM-9 and AF-10 cells. However, the enhancement of plasma cell responses by GM1 was specific and was not mediated by IL-6, since GM1 activity was blocked by anti-GM1 monoclonal antibody (mAb), but not by control IgM, anti-IL-6 Ab or the anti-IL-6 receptor mAb, PM1. Conversely, the enhancement by IL-6 was blocked by anti-IL-6 Ab and PM1, but not by anti-GM1 mAb. GM1, but not other gangliosides, also enhanced Ig production and proliferation in freshly separated plasma cells from patients with plasma cell leukemia and in plasma cells generated in vitro. These actions of GM1 were specifically blocked by anti-GM1 mAb, but not by anti-IL-6 Ab or PM1. These results indicate that GM1 may be an important regulator of plasma cell responses.
研究了神经节苷脂对人浆细胞反应的影响。在测试的各种神经节苷脂中，只有GM1增强了人浆细胞系IM-9和AF-10中免疫球蛋白（Ig）的产生和增殖，而其他神经节苷脂（GM2、GM3、GD1a、GD1b、GD3、GT1b和GQ1b）则没有作用。在测试的各种细胞因子中，包括白细胞介素（IL）-1β、IL-2、IL-3、IL-4、IL-5、IL-6、IL-9、IL-10、IL-12、IL-13、干扰素（IFN）-α和IFN-γ，只有IL-6增强了IM-9和AF-10细胞中Ig的产生和增殖。然而，GM1对浆细胞反应的增强是特异性的，且不是由IL-6介导的，因为GM1的活性被抗GM1单克隆抗体（mAb）阻断，但不被对照IgM、抗IL-6抗体或抗IL-6受体mAb PM1阻断。相反，IL-6的增强作用被抗IL-6抗体和PM1阻断，但不被抗GM1 mAb阻断。GM1而非其他神经节苷脂，也增强了浆细胞白血病患者新鲜分离的浆细胞以及体外产生的浆细胞中Ig的产生和增殖。GM1的这些作用被抗GM1 mAb特异性阻断，但不被抗IL-6抗体或PM1阻断。这些结果表明GM1可能是浆细胞反应的重要调节因子。
