Hinterberg K, Scherf A, Gysin J, Toyoshima T, Aikawa M, Mazie J C, da Silva L P, Mattei D
Unité de Parasitologie Expérimentale URA361, Institut Pasteur, Paris, France.
Exp Parasitol. 1994 Nov;79(3):279-91. doi: 10.1006/expr.1994.1091.
The transport of the megadalton protein Pf332 was studied during the asexual bloodstage development of Plasmodium falciparum. Four mouse monoclonal antibodies, produced against a recombinant polypeptide derived from the Pf332 protein, were used to analyze the kinetics of synthesis, the subcellular location, and transport of this giant molecule to the erythrocyte membrane. After parasite invasion of a red blood cell, the Pf332 antigen is first detected in young trophozoites at the parasitophorous vacuole membrane or in the cytoplasm of the erythrocyte as large vesicle-like structures. The number of vesicles increases during maturation of the parasite and thus forms a rim-like immunofluorescence pattern between the erythrocyte membrane and the parasitophorous vacuole at very late stages. The various anti-Pf332 antibodies react with the surface of erythrocytes infected with very mature parasites (segmenter stage 42-46 hr postinvasion). Immunoelectron microscopic analysis shows that the Pf332 antigen is transported in association with Maurer's clefts in the cytoplasm of the erythrocyte. This transport could be completely blocked by Brefeldin A, resulting in the accumulation of the antigen within the parasite. These data strongly suggest that the Pf332 antigen is exported to the erythrocyte cytoplasm via the classical Golgi secretory pathway.
在恶性疟原虫无性血液阶段发育过程中，对兆道尔顿蛋白Pf332的转运进行了研究。使用针对源自Pf332蛋白的重组多肽产生的四种小鼠单克隆抗体，来分析这种巨分子的合成动力学、亚细胞定位以及向红细胞膜的转运。疟原虫侵入红细胞后，Pf332抗原首先在年轻滋养体的寄生泡膜或红细胞胞质中以大的囊泡样结构被检测到。在疟原虫成熟过程中，囊泡数量增加，因此在非常晚期阶段在红细胞膜和寄生泡之间形成边缘样免疫荧光模式。各种抗Pf332抗体与感染了非常成熟疟原虫（侵入后42 - 46小时的裂殖体阶段）的红细胞表面发生反应。免疫电子显微镜分析表明，Pf332抗原与红细胞胞质中的毛雷尔氏小体相关联进行转运。这种转运可被布雷菲德菌素A完全阻断，导致抗原在疟原虫内积累。这些数据强烈表明，Pf332抗原通过经典的高尔基体分泌途径输出到红细胞胞质中。
