一种编码具有蔗糖分解活性的新型植物酶的互补DNA的特性分析。

Characterisation of a complementary DNA encoding a novel plant enzyme with sucrolytic activity.

作者信息

Machray G C, Burch L, Hedley P E, Davies H V, Waugh R

机构信息

Cell and Molecular Genetics Department, Scottish Crop Research Institute, Invergowrie, Dundee, UK.

出版信息

FEBS Lett. 1994 Oct 31;354(1):123-7. doi: 10.1016/0014-5793(94)01105-2.

DOI:10.1016/0014-5793(94)01105-2

PMID:7957893

Abstract

The cloning of a 1332 bp cDNA from a potato (Solanum tuberosum L.) cv. Cara leaf cDNA expression library, using an antibody raised against a purified tuber protein preparation with sucrolytic activity, is described. The corresponding gene in potato is of low copy number, is expressed in a variety of tissues, and encodes a protein which includes several domains with similarity to database sequences, including ferredoxin from Clostridium pasteurianum. Expression of the cDNA in E. coli yields a fusion protein with sucrolytic activity.

摘要

本文描述了利用针对具有蔗糖分解活性的纯化块茎蛋白制剂产生的抗体，从马铃薯（Solanum tuberosum L.）品种Cara叶片cDNA表达文库中克隆出一个1332 bp的cDNA。马铃薯中的相应基因拷贝数低，在多种组织中表达，并编码一种蛋白质，该蛋白质包含几个与数据库序列相似的结构域，包括来自巴氏梭菌的铁氧还蛋白。该cDNA在大肠杆菌中的表达产生了具有蔗糖分解活性的融合蛋白。