Hedley P E, Machray G C, Davies H V, Burch L, Waugh R
Scottish Crop Research Institute, Dundee, UK.
Plant Mol Biol. 1993 Aug;22(5):917-22. doi: 10.1007/BF00027378.
A cDNA clone encoding an invertase isoenzyme has been isolated from a potato leaf cDNA library. The deduced amino acid sequence shows significant similarities to previously characterised invertases. The highest degree of overall similarity, including the signal peptide sequence, is to carrot cell wall invertase, suggesting that the potato gene encodes an apoplastic enzyme. Expression of the gene, as determined by RT-PCR, is detected in stem and leaf tissue, and at lower levels in tuber, but is absent from roots.
已从马铃薯叶片cDNA文库中分离出一个编码转化酶同工酶的cDNA克隆。推导的氨基酸序列与先前鉴定的转化酶具有显著相似性。包括信号肽序列在内的总体相似性最高的是胡萝卜细胞壁转化酶，这表明马铃薯基因编码一种质外体酶。通过RT-PCR测定，该基因在茎和叶组织中表达，在块茎中的表达水平较低，但在根中不表达。
