Hanzawa H, Haruyama H, Watanabe K, Tsurufuji S
Analytical and Metabolic Research Laboratories, Sankyo Co. Ltd., Tokyo, Japan.
FEBS Lett. 1994 Nov 7;354(2):207-12. doi: 10.1016/0014-5793(94)01081-1.
The solution conformation of rat cytokine-induced neutrophil chemoattractant (CINC/Gro), a small protein consisting of 72 amino acid residues with proinflammatory activities, and a member of the interleukin 8 family corresponding to a counterpart of human Gro, was investigated with homonuclear 2D and 3D NMR spectroscopy. At each phase of the structural analysis, the homonuclear 3D NOESY-HOHAHA and HOHAHA-NOESY spectra afforded valuable data, removing ambiguities intractable by conventional 2D NMR techniques. CINC/Gro exists as a dimer in solution and contains a triple stranded anti-parallel beta-sheet and C-terminal alpha-helix in the monomer structure, as observed in human IL-8, but non-trivial differences are also observed.
