通过核磁共振光谱法对人黑素瘤生长刺激活性（MGSA）进行¹H归属和二级结构测定。

1H assignment and secondary structure determination of human melanoma growth stimulating activity (MGSA) by NMR spectroscopy.

作者信息

Fairbrother W J, Reilly D, Colby T, Horuk R

机构信息

Department of Protein Engineering, Genentech Inc., South San Francisco, CA 94080-4990.

出版信息

FEBS Lett. 1993 Sep 20;330(3):302-6. doi: 10.1016/0014-5793(93)80893-y.

DOI:10.1016/0014-5793(93)80893-y

PMID:8397104

Abstract

The solution structure of melanoma growth stimulating activity (MGSA) has been investigated using proton NMR spectroscopy. Sequential resonance assignments have been carried out, and elements of secondary structure have been identified on the basis of NOE, coupling constant, chemical shift, and amide proton exchange data. Long-range NOEs have established that MGSA is a dimer in solution. The secondary structure and dimer interface of MGSA appear to be similar to those found previously for the homologous chemokine interleukin-8 [Clore et al. (1990) Biochemistry 29, 1689-1696]. The MGSA monomer contains a three stranded anti-parallel beta-sheet arranged in a 'Greek-key' conformation, and a C-terminal alpha-helix (residues 58-69).

摘要

已使用质子核磁共振光谱法研究了黑素瘤生长刺激活性（MGSA）的溶液结构。已进行了序列共振归属，并根据核Overhauser效应（NOE）、耦合常数、化学位移和酰胺质子交换数据确定了二级结构元件。长程NOE已证实MGSA在溶液中是二聚体。MGSA的二级结构和二聚体界面似乎与先前在同源趋化因子白细胞介素-8中发现的相似[Clore等人（1990年）《生物化学》29，1689 - 1696]。MGSA单体包含以“希腊钥匙”构象排列的三股反平行β折叠片，以及一个C端α螺旋（残基58 - 69）。