Gicquel B
Unité de Génétique Mycobactérienne, Institut Pasteur, Paris, France.
Dev Biol Stand. 1994;82:171-8.
BCG, the Bacillus of Calmette and Guérin, has been used as a vector for the presentation of foreign antigens. The nef gene of HIV was cloned and expressed in BCG. A specific cellular immune response against Nef was observed following inoculation of mice. As a model system beta-galactosidase has also been expressed in BCG. Antibody responses against beta-galactosidase in addition to cellular responses were observed. Mucosal immunity was demonstrated after oral immunization of mice. With the aim of isolating new vaccines against tuberculosis, transposon mutagenesis was investigated. By using Tn610, representative libraries of Mycobacterium smegmatis insertion mutants were constructed.
卡介苗（Calmette和Guérin芽孢杆菌）已被用作呈递外源抗原的载体。HIV的nef基因被克隆并在卡介苗中表达。接种小鼠后观察到针对Nef的特异性细胞免疫反应。作为模型系统，β-半乳糖苷酶也已在卡介苗中表达。除细胞反应外，还观察到针对β-半乳糖苷酶的抗体反应。小鼠口服免疫后证明了黏膜免疫。为了分离新型抗结核疫苗，对转座子诱变进行了研究。通过使用Tn610，构建了耻垢分枝杆菌插入突变体的代表性文库。
