Rhodes G H, Abai A M, Margalith M, Kuwahara-Rundell A, Morrow J, Parker S E, Dwarki V J
Vical Inc., San Diego, CA.
Dev Biol Stand. 1994;82:229-36.
Antibodies to the influenza virus nucleoprotein are produced after injection of a plasmid DNA expression vector containing the nucleoprotein gene. A single injection of 10 to 100 micrograms of DNA produces IgG antibodies which first appear four to six weeks post injection. Antibody titres peak at six to eight weeks and then remain stable in most animals for many months. The antibody response at early times is dose-dependent, with higher amounts of DNA producing a faster immune response as well as higher antibody titres. Titres observed many months after injection are less dependent on DNA dose.
注射含有核蛋白基因的质粒DNA表达载体后，会产生针对流感病毒核蛋白的抗体。单次注射10至100微克DNA会产生IgG抗体，这些抗体在注射后四至六周首次出现。抗体滴度在六至八周达到峰值，然后在大多数动物体内保持稳定数月。早期的抗体反应呈剂量依赖性，较高剂量的DNA会产生更快的免疫反应以及更高的抗体滴度。注射数月后观察到的滴度对DNA剂量的依赖性较小。
