Kelly S M, Pitcher M C, Farmery S M, Gibson G R
Department of Gastroenterology, Addenbrooke's Hospital, Cambridge, England.
Gastroenterology. 1994 Dec;107(6):1671-4. doi: 10.1016/0016-5085(94)90806-0.
BACKGROUND/AIMS: Epidemiological studies suggest that fecal-oral spread of Helicobacter pylori potentially represents an important route of infection. However, the bacterium has never been isolated from feces of adults in the developed world. This study attempted to isolate H. pylori from stool specimens donated by 36 adults from the United Kingdom who had dyspepsia.
Fresh fecal samples were obtained and, after centrifugation to harvest bacteria, cultured onto H. pylori selective growth media. Pure colonies of H. pylori were obtained by subculture and were then analyzed using polymerase chain reaction to confirm genotypic identity.
H. pylori was isolated from feces in 12 of 25 subjects with dyspepsia proven to be H. pylori-positive at endoscopy and/or 14C urea breath test. Initial bacterial identification was made on the basis of various phenotypic methods. Genotypic confirmation that the bacterium was indeed H. pylori was also made.
This study is the first to conclusively show that H. pylori can be isolated from feces obtained from adults in the United Kingdom. The implication of this finding is that transmission of H. pylori infection by the fecal-oral route is feasible.
背景/目的：流行病学研究表明，幽门螺杆菌的粪-口传播可能是一条重要的感染途径。然而，在发达国家，从未从成年人粪便中分离出该细菌。本研究试图从36名来自英国的患有消化不良的成年人捐赠的粪便标本中分离幽门螺杆菌。
获取新鲜粪便样本，离心收集细菌后，接种于幽门螺杆菌选择性生长培养基上。通过传代培养获得幽门螺杆菌纯菌落，然后用聚合酶链反应进行分析以确认基因型。
在内镜检查和/或¹⁴C尿素呼气试验证实为幽门螺杆菌阳性的25名消化不良患者中，有12人的粪便中分离出了幽门螺杆菌。最初通过各种表型方法进行细菌鉴定。同时也进行了基因型确认，证明该细菌确实是幽门螺杆菌。
本研究首次确凿地表明，在英国可从成年人粪便中分离出幽门螺杆菌。这一发现意味着幽门螺杆菌通过粪-口途径传播是可行的。
